Figure 4

BiFC visualization of P6274-703 interaction in agrobacterium-infiltrated N. benthamiana leaves. Co-expression of P6274-703-YN and P6274-703-YC induced strong recovered YFP signals in the cytoplasm, and no YFP signals were detected for the negative controls following the co-expression of P6274-703-YN/YC or P6274-703-YC/YN. YFP was excited at 488 nm and emission was measured at 550-590 nm. The fluorescent and bright field images are depicted in the upper and lower panels, respectively. Bars, 20 μm.