P6 forms punctate, cytoplasmic VLS in the onion epidermal cells and self-interacts in YTH system. (A) Subcellular localization of RBSDV P6 fused to GFP and free GFP in onion epidermal cells. Punctata VLS of different sizes were prevalently formed in the onion cells expressing P6-GFP, while diffuse GFP fluorescence was observed in the nucleus and cytoplasm of the cells expressing free GFP. The results were observed 16-24 h after particle bombardment. Bars, 50 μm. (B) Yeast colonies containing pGBKT7-P6/pGADT7-P6 grew well on the selective medium as did yeast colonies containing pGBKT7-T/pGADT7-p53, which was used as the positive control, whereas yeast transformed with pGBKT7-P6/pGADT7 or pGBKT7/pGADT7-P6 used as negative controls were unable to grow.