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Table 1 Summary of the symptoms induced by the expression of ACMV, CLCuMV, CbLCuV and TYLCV genes from a PVX vector in N. benthamiana

From: Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in Nicotiana benthamianaand their correlation with the levels of developmental miRNAs

Gene

Construct

Latent periodâ–¡(days)

Recoveryâ–³

(dpi)â–²

Symptoms#

-

PVX

7

Yes(12-14)

MVY, VT, MMo,

gfp

PVX- gfp

6

Yes (12-14)

MVY, VT, Mo and GFP

 

PVX-Mu-rep

7

Yes (14-16)

Nc, LCu, NcO, NcS

rep

PVX-A-rep

7

No

NcO, SLCu, VY, NcS

 

PVX-Cb-rep

7

Yes (14-16)

NcO, MLcu, NcS

 

PVX-T-rep

nd*

nd*

nd*

 

PVX-Mu-c2

7

Yes (14-16)

NcO, MLCu, NcS

trap/c2

PVX-A-trap

5

Yes (12-14)

VY, VNc, MLCu

 

PVX-Cb-trap

7

Yes (14-16)

VY, VNc, MLCu

 

PVX-T-c2

7

Yes (14-16)

IVC

 

PVX-Mu-ren

7

Yes (14-15)

MVY, VT, M Mo

ren

PVX-A-ren

7

Yes (14-15)

MVY, VT, M Mo

 

PVX-Cb-ren

7

Yes (14-15)

MVY, VT, M Mo

 

PVX-T-ren

7

Yes (14-15)

MVY, VT, M Mo

 

PVX-Mu-c4

7

No

MLCu, VY, DL

(a)c4

PVX-A-ac4

7

Yes (14-16)

MVY, VT, M Mo

 

PVX-Cb-ac4

7

Yes (14-16)

VY, LCr, VS

 

PVX-T-c4

7

Yes (14-16)

Et, MLCu,

 

PVX-Mu-cp

7

Yes (14-15)

MVY, VT, M Mo.,

cp

PVX-A-cp

7

Yes (14-15)

MLCu, Mos

 

PVX-Cb-cp

7

Yes (14-15)

MVY, VT, MMo

 

PVX-T-cp

7

Yes (14-15)

LCr, LCu, Mos

 

PVX-Mu-v2

6

No

NcO, SLCu, VNc,

(a)v2

PVX-A-av2

8

Yes (16-18)

LCu, VY

 

PVX-T-v2

7

No

SLCu, St, VY, NcS

mp

PVX-A-mp

7

Yes (14-15)

MVY, VT, M Mo,

 

PVX-Cb-mp

7

Yes (14-15)

MVY, VT, M Mo,

nsp

PVX-A-nsp

7

Yes (14-15)

MLCu, NcS

 

PVX-Cb-nsp

7

Yes (14-15)

MVY, VT, M Mo

c5

PVX-Ko-c5

8

Yes (14-16)

NcO, Mos,

βc1

PVX-Mu- βC1

6

No

SLCu, St, En, VS, VD

  1. *Not determined.
  2. â–³Newly developing leaves showing no symptoms.
  3. â–²Approx. number of days after inoculation that symptom reversion (recovery) started.
  4. â–¡Period between inoculation and first sign of appearance of symptoms.
  5. #Symptoms are indicated as mild vein yellowing (MYV), vein thickening (VT), mild mosaic (MMo), necrosis (Nc), leaf curling (LCu), necrosis at site of inoculation (NcO), systemic necrotic lesions (NcS), severe leaf curling (SLCu), Vein yellowing (VY), mild leaf curling (MLCu), veinal necrosis (VNc), inter veinal chlorosis (IVC), deformed leaves (DL), leaf crumpling (LCr), etiolation (Et), mosaic (Mos), stunting (St), enations (En), vein swelling (VS) and vein darkening (VD).