Figure 5From: Characterization of duck enteritis virus UL53 gene and glycoprotein KExpression kinetics of DEV UL53 gene and intracellular location of the DEV gK in infected DEF by IIF. A. Proteins isolated from mock or DEV-infected cells at different times (10 h.p.i. to 48 h.p.i.) were analyzed by Western blot. The specific band demonstrated the expected position for DEV gK (about 38 kDa). M represented standard protein molecular weight markers. B. Expression kinetics and intracellular location of the DEV gK in infected DEF. Mock-infected(a) and DEV infected DEF(b to f) were fixed as described in Methods. The samples were incubated with the purified rabbit anti-UL53 protein polyclonal antibodies(a, c to f) or preimmune serum(b), and reacted with fluorescein isothiocyanate (FITC)-conjugated secondary antibody, and then counter-stained with DAPI (the cell nuclei was stained with blue). The merged fluorescence microscopy images of DEF are shown from a to f with high magnification (400 ×).Back to article page