Expression kinetics of DEV UL53 gene and intracellular location of the DEV gK in infected DEF by IIF. A. Proteins isolated from mock or DEV-infected cells at different times (10 h.p.i. to 48 h.p.i.) were analyzed by Western blot. The specific band demonstrated the expected position for DEV gK (about 38 kDa). M represented standard protein molecular weight markers. B. Expression kinetics and intracellular location of the DEV gK in infected DEF. Mock-infected(a) and DEV infected DEF(b to f) were fixed as described in Methods. The samples were incubated with the purified rabbit anti-UL53 protein polyclonal antibodies(a, c to f) or preimmune serum(b), and reacted with fluorescein isothiocyanate (FITC)-conjugated secondary antibody, and then counter-stained with DAPI (the cell nuclei was stained with blue). The merged fluorescence microscopy images of DEF are shown from a to f with high magnification (400 ×).