Transcript detection of the UL53 by nucleic acid inhibition test. A. The integrality of RNA samples tested by agarose gel electrophoresis. Total RNA was isolated three bands(28S, 18S and 5S). B. Detection of the UL53 transcript by nucleic acid inhibition test. M: DNA marker--Marker I. DEF were infected with DEV in the absence (Mock) or presence of 300 μg/ml ganciclovir and total RNA of infected cells were harvested at 24 h and 36 h postinfection followed by RT-PCR detection using specific primers to UL53 gene and β-actin gene respectively.