FQ-RT-PCR was used to detect the transcript of UL53 gene. A. The amplification curves of DEV UL53 gene and β-actin gene to detect the samples by FQ-RT-PCR. B. Gene expression analysis of UL53 gene by iQ™5 Optical System Software Version 2.1. The x-axis represented the conditions of different infected time, and the y-axis represented UL53 gene relative expressing quantity.