Figure 2
From: Characterization of duck enteritis virus UL53 gene and glycoprotein K
The standard curves and melt curves of DEV UL53 gene and β-actin gene by FQ-RT-PCR. A: Standard curve of DEV UL53 gene. The x-axis represented ten-fold dilutions of pET32b/UL53 plasmids, and the y-axis represented corresponding cycle threshold (Ct) values. The standard curve equation is Y = -3.305X-0.641. The correlation coefficient and the slope value of the regression curve were 0.998 and -3.305, respectively; B: Melt curve of DEV UL53 gene. There was only a singlet; C: Standard curve of β-actin gene. The x-axis represented ten-fold dilutions of pMD18-T/β-actin plasmids, and the y-axis represented corresponding cycle threshold (Ct) values. The standard curve equation is Y = -3.358X+6.066. The correlation coefficient and the slope value of the regression curve were 0.999 and -3.358, respectively; D: Amplification curve of β-actin gene. There was only a singlet, too.