Figure 4
From: Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells
Protein and mRNA assays for the detection of p53, p21, p27, p16, cyclinD1, cyclinE, Rb and pRb. (A) Immunohistochemistry assay (400×). Bar, 50 μm. All proteins were located in nuclei and a positive result was brown nuclear staining. (B) Western blot analysis. (C) Semi-quantitative RT-PCR analysis. (D) The relative expression level of each protein was represented as the fold ratio. Compared with HepG2 cells. *p < 0.05, **p < 0.01.