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Table 5 Testing results of duplex real-time PCR assay and singleplex real-time PCR assay for 40 serum samples

From: Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection

Duplex primer/probe assay Singleplex primer/probe assay (set A primer/probe) Singleplex primer/probe assay (set B primer/probe) Number of samples detected
+ + + 28
+ - - 2
- - - 10
  1. +: positive result
  2. -: negative result