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Table 5 Testing results of duplex real-time PCR assay and singleplex real-time PCR assay for 40 serum samples

From: Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection

Duplex primer/probe assay

Singleplex primer/probe assay (set A primer/probe)

Singleplex primer/probe assay (set B primer/probe)

Number of samples detected

+

+

+

28

+

-

-

2

-

-

-

10

  1. +: positive result
  2. -: negative result