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Table 2 Optimization of the concentration of IC

From: Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection

Armored DNA concentration (copies/ml) National reference material 4 for HBV DNA 5 × 105 IU/ml National reference material 3 for HBV DNA 5 × 104 IU/ml National reference material 2 for HBV DNA 5 × 103 IU/ml National reference material 1 for HBV DNA 5 × 102IU/ml HBV 0 IU/ml
  IC (Cy5)Ct HBV (FAM)Ct IC (Cy5)Ct HBV (FAM)Ct IC (Cy5)Ct HBV (FAM)Ct IC (Cy5)Ct HBV (FAM)Ct IC (Cy5)Ct
100 000 38.92 28.00 34.43 32.17 31.18 35.78 30.33 > 45 30.13
10 000 41.51 28.93 38.39 31.20 35.58 34.29 34.29 39.55 34.21
1 000 > 45 23.14 38.35 26.44 37.72 29.23 35.68 32.67 35.47
0 > 45 23.21 > 45 26.11 > 45 29.14 > 4 5 32.82 > 45
  1. Concentrations of HBV DNA and armored DNA were indicated by FAM and Cy5 signals, respectively.