Table 2 Optimization of the concentration of IC
Armored DNA concentration (copies/ml) | National reference material 4 for HBV DNA 5 × 105 IU/ml | National reference material 3 for HBV DNA 5 × 104 IU/ml | National reference material 2 for HBV DNA 5 × 103 IU/ml | National reference material 1 for HBV DNA 5 × 102IU/ml | HBV 0 IU/ml | ||||
|---|---|---|---|---|---|---|---|---|---|
| Â | IC (Cy5)Ct | HBV (FAM)Ct | IC (Cy5)Ct | HBV (FAM)Ct | IC (Cy5)Ct | HBV (FAM)Ct | IC (Cy5)Ct | HBV (FAM)Ct | IC (Cy5)Ct |
100 000 | 38.92 | 28.00 | 34.43 | 32.17 | 31.18 | 35.78 | 30.33 | > 45 | 30.13 |
10 000 | 41.51 | 28.93 | 38.39 | 31.20 | 35.58 | 34.29 | 34.29 | 39.55 | 34.21 |
1 000 | > 45 | 23.14 | 38.35 | 26.44 | 37.72 | 29.23 | 35.68 | 32.67 | 35.47 |
0 | > 45 | 23.21 | > 45 | 26.11 | > 45 | 29.14 | > 4 5 | 32.82 | > 45 |