Figure 1

Linearity of the duplex real-time PCR assay. Linearity of the duplex real-time PCR assay was determined using serial 10-fold dilutions of HBV standard in negative serum samples at the following concentrations: 10, 10², 10³, 10⁴, 10⁵, and 10⁶ IU/ml. Three replicates were tested in a single run at each concentration. Linear relationship between the Ct values and the log10 HBV DNA concentration in the samples at concentrations from 10 to 106 IU/ml yielded R = 0.993.