Figure 3

Anti-HCV activity of acetonic and n-hexane fractions of AN: Huh-7 cells were incubated with HCV serum and 50 μg/μl concentration of the herbal extract in different solvents for 24 hours. At the end of incubation period, total RNA was extracted by Gentra kit, and the levels of HCV RNA remaining were determined by the Quantitative RT-PCR assay and are shown as a percentage relative to the levels of HCV RNA in cells incubated without compound (control).