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Table 1 Specific primers for amplification/sequencing of Araraquara hantavirus nucleoprotein gene and amplification of betagalactosidase gene.

From: Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli

Primers

name

Primer

Sequence

Function

For. His.

5' GAAGATCTATGCGGGGTTCTCATCAT 3'

Amplification of N gene.

Rev. NP.

5 TAACCCGGGTCACAGCTTTAAGGGTCC 3'

Amplification of N gene.

Int. NP.

5' AGACAGCAGACTGGAAG 3'

Sequencing of N gene.

For. pSyn

5' GGGCCAAGCTTGGCGTTATTG 3'

Sequencing of N gene

Rev. pSyn

5' TCTGTAAATCAACAACGCACAG 3'

Sequencing of N gene

For. β-gal

5' TTCACTGGCCGTCGTTTTACAACGTCGTGA 3'

Check of Recombinant Baculovírus Purity

Rev. β-gal

5' ATGTGAGCGAGTAACAACCCGTCGGATTCT3'

Check of Recombinant Baculovírus Purity

  1. Primers name: For. His - Forward Histidina; Rev. NP - Reverse Nucleoprotein; Int. NP. Intermediate Nucleoprotein; For. pSyn. Forward vector pSyn; Rev. pSyn. Reverse vector pSyn; For. β-gal. Forward gene betagalactosidase; Rev. β-gal. Reverse gene betagalactosidase.
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Virology Journal

ISSN: 1743-422X