Detection of NS3 in mouse brain cells. One-week-old sucking mice were infected with the SH-JEV01 strain of JEV (1.0 × 106 PFU). Brain samples were collected from mice showing neurological signs and stained immunohistochemically with the anti-NS3 antibody. The nuclei were counter-stained by hematoxylin. Brain samples from the mock-infected mice (Mock) were used as controls. (A) Immunohistochemical examination of the cerebral cortex. Bar, 50 μm. (B) Immunohistochemical examination of the cerebellum. Bar, 200 μm. Arrows indicate the NS3-positive cells.