Figure 1
From: Enhancement of bacteriolysis of Shuffled phage PhiX174 gene E
Physical map of prokaryotic non-fusion expression vector pBV220. Ampr, ampicillin resistance gene; ori, replication origin; cIts857, restraining gene of lambda bacteriophage adapted to heat induced expression; PRPL, tandem promoters of lambda bacteriophage for the high level expression; SD, Shine-Dalgarno sequence; rrnB, ribosome rrnB gene providing translation stop signal; and Eco RI and Bam HI, restriction enzyme sites to insert mutant lysis E gene into vector.