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Table 1 Intra-assay performance of TaqMan qPCR with five different Chinese strains of CSFV

From: Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs

Samples CT value Result of intra-assay
  1 2 3 4 5 6 7 8 9 Mean SDs CVs (%)
BJ1/08 21.23 21.39 21.42 21.43 21.53 21.60 23.10 23.14 23.17 21.67 0.366 1.62
BJ2/08 18.01 18.09 18.16 18.17 18.19 18.55 18.56 18.79 18.89 18.38 0.324 1.76
HeNXH2/98 21.11 21.14 21.17 21.40 21.44 23.02 23.17 23.28 23.55 21.70 0.557 2.45
FJFQ1/98 15.99 16.06 16.15 16.19 16.21 16.35 16.16 17.24 17.25 17.47 0.489 2.80
HeNBY1/96 24.70 25.04 25.06 25.41 25.05 25.81 25.82 26.29 26.39 26.42 0.599 2.27
  1. 9 repeats assay performed at the same time and under the same conditions
  2. SD = standard deviation
  3. CV = coefficient of variation