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Figure 1 | Virology Journal

Figure 1

From: Deletion of the meq gene significantly decreases immunosuppression in chickens caused by pathogenic marek's disease virus

Figure 1

Immunofluorescence analysis of CEF cells infected with recombinant viruses. 100 PFU of bac-GX0101 and GX0101ΔMeq were inoculated into 6-well plates containing a monolayer of CEFs. The mAb H19 specific for the MDV-unique protein pp38, and mouse serum against Meq were used for immunofluorescence analysis. Parental virus, bac-GX0101 expressed Meq protein, whereas the deletion mutant virus GX0101ΔMeq did not. The presence of GX0101ΔMeq virus was confirmed by staining of MDV-specific pp38 protein.

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