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Table 1 Descriptions of used plasmids

From: Tetherin inhibits prototypic foamy virus release

Name

Descriptions

Flag-HuTHN, Flag-AgmTHN, Flag-BovTHN, Flag-CanTHN

Human, African green monkey, bovine and canine tetherin expression plasmids

Flag-HuTHN delCT,

Human tetherin 20-180 aa expression plasmid

Flag-HuTHN delTM

Human tetherin 47-180 aa expression plasmid

Flag-HuTHN delGPI

Human tetherin 1-161 aa expression plasmid

Flag-AgmTHN delCT

African green monkey tetherin 26-182 aa expression plasmid

Flag-AgmTHN delTM

African green monkey tetherin 50-182 aa expression plasmid

Flag-AgmTHN delGPI

African green monkey tetherin 1-159 aa expression plasmid

HuTHN C53A, HuTHN C63A, HuTHN C91A

Human tetherin single cysteine mutated to alanine

HuTHN C53/91A, HuTHN C53/63A, HuTHN C63/91A

Human tetherin two of the three cysteines mutated to alanines

HuTHN C53/63/91A

Human tetherin all three cysteines mutated to alanines

HuTHN N2A

Human tetherin two asparagines at 65 and 92 mutated to alanines

HuTHN C3A/N2A

Human tetherin all three cysteines and two asparagines mutated to alanines