Tetherins inhibit the release of PFV in a dose-dependent manner. Both the TM domain and GPI anchor of AgmTHN are important for the inhibition to PFV. (A-C) pcPFV was transfected into 293T cells together with the indicated 10, 20, 50, 100 ng of BovTHN (A), CanTHN (B), or AgmTHN (C). The titer of virus in supernatants was analyzed by PFVL. (D) 293T cells were transfected with pcPFV, together with the indicated AgmTHN wild type or truncation mutants. WT, wild type AgmTHN; delCT, AgmTHN (26-182 aa); delTM, AgmTHN (50-182 aa); delGPI, AgmTHN (1-159 aa). The titer of virus in supernatants was analyzed by infecting PFVL.