Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Tetherin inhibits prototypic foamy virus release

Figure 3

The dimerization and glycosylation of human tetherin are not essential for its antiviral activity against PFV. (A) Schematic representations of tetherin and various cysteine or asparagine to alanine mutants. (B) WT and mutant tetherin expressed in 293T cells were analyzed under both reducing and non-reducing conditions by Western blotting (*, non-specific band). (C) pcPFV was transfected into 293T cells together with the indicated human tetherin wild type or mutants. The titer of virus in supernatants was analyzed by infecting PFVL. (D-F) 293T cells were transfected with pcPFV and indicated 10, 20, 50, 100 ng of HuTHN mutants C53/63/91A (D), N65/92A (E), or C3A/N2A (F). The titer of virus in supernatants was analyzed by infecting the PFVL indicator cells.

Back to article page