Figure 3

Inflammation in the brain of VEEV infected IKO and WT mice. (a) IKO 96 hr pi cerebrum, subventricular zone, H&E, 400X: There were low numbers of necrotic neurons, characterized by small amounts of karyorrhexis (arrows), when compared to the same region of the WT mice (b). (b) WT 96n hr pi, cerebrum, subventricular zone, H&E, 400X: There were high numbers of necrotic neurons, characterized by large amounts of karyorrhexis (arrows), when compared to the same region of IKO mice (a). (c) IKO 96 hr pi, brainstem, H&E, 400X: There was little to no endothelial swelling and no perivascular cuffing (arrows) compared to the WT mice (d). (d) WT 96 hr pi, brainstem, H&E, 400X: There was moderate endothelial swelling and mild perivascular cuffing (thick arrows) and rare necrotic cells (thin arrow) compared to the IKO mice (c). (e) IKO 96 hr pi, brain, thalamus, H&E, 400X: There was little to no endothelial swelling and no perivascular cuffing (arrows) compared to the WT mice (f). (f) WT 96 hr pi, brain, thalamus, H&E, 400X: There was moderate endothelial swelling and mild perivascular cuffing (thick arrow) and rare necrotic cells (thin arrows) compared to the IKO mice (e).