Spry1 co-localizes with E1A
in the cytoplasm. The subcellular localization of Spry1 and E1A13s was determined in HeLa cells by confocal immunofluorescence microscopy using antibodies that specifically recognize Spry1 (green) and the Myc-tagged E1A13s (red) protein constructs. (A) Spry1 and (B) E1A13s transfected cells were left serum starved overnight and stimulated with 20 ng/ml bFGF for 2 h. (C) Images were overlaid for comparison of staining, yellow indicates co-localization of the two proteins. Scale bar, 10 μm.