Spry1 interacts with E1A
of the non-oncogenic Adenovirus type 2 (Ad2) and with the Human papillomavirus type 16 (HPV16) E7 protein. Lysates from cell extracts expressing Spry1 were incubated with (A) GST-Ad2-E1A13S and (B) GST-HPV16-E7-fusion proteins or for control reactions with the leader sequence GST or GST-Ad12-E1A13S as indicated. The assays were performed with 0.5 mg of cellular lysate and 40 μg of GST-fusion proteins at 4°C for 1 h. The bound proteins were subjected to SDS-PAGE and analyzed by immunoblotting. Spry1 was detected by an antibody directed against the Myc-epitope of the Spry-fusion protein.