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Table 3 Mutations observed in antibody-resistant isolates.

From: "Boom" and "Bust" cycles in virus growth suggest multiple selective forces in influenza a evolution

Gene [segment]
(reference sequence, accession)
CalX wt
Change: AA [NT]
P2
Change: AA [NT]
P16A1
Change: AA [NT]
PB2 [segment 1] (A/Puerto Rico/8/34, V00603.1)13 G309D [G953A], Y360S [A1106C], [C1540A], [G1815A] G309D [G953A], Y360S [A1106C], [C1540A], [G1815A], A661V [C2009T]14 G309D [G953A], Y360S [A1106C], [C1540A], [G1815A], A661V [C2009T], E676D [A2058C]15
PB1 [segment 2] (A/Puerto Rico/8/34, J02151.1) A53G [C282G], [T504A], S216G [A670G], [C730T]16, L300F [G924C], H473L [A1442T], S517I [T1563G], [T1574G] A53G [C282G], [T504A], S216G [A670G], L300F [G924C], H473L [A1442T], S517I [T1563G], [T1574G] A53G [C282G], [T504A], S216G [A670G], L300F [G924C], H473L [A1442T], S517I [T1563G], [T1574G]
PA [segment 3] (A/Puerto Rico/8/34, V01106.1)13 K158R [A497G], K391E [A1194G], [T1308A] K158R [A497G], [T1308A] K158R [A497G], [A783G], [T1308A]
HA [segment 4] (A/California/07/2004, EU103820.1) 100% homology 100% homology 100% homology
NP [segment 5] (A/Puerto Rico/8/34, J02147.1)13 [G306A], D247N [G784A] [G306A], D247N [G784A] Failed to amplify17
NA [segment 6] (A/California/07/2004, EU103978.1) 100% homology 100% homology V240I [G718A]
M1 &M2 [segment 7] (A/Puerto Rico/8/34, V01099.1) 100% homology 100% homology 100% homology
NS1 & NS2/NEP [segment 8] (A/Puerto Rico/8/34, J02150.1) 100% homology 100% homology 100% homology
  1. 13 CalX wt and antibody-selected isolates most closely related to A/reassortant/NIBRG-14(Viet Nam/1194/2004 × Puerto Rico/8/1934)(H5N1).
  2. 14 Mutations present in P2 and/or P16 but not in wild type are shown in bold type
  3. 15 Present as a mixture with wild type sequence at a mutant:wildtype ratio of approximately 1:2 (see Figure S3).
  4. 16 Mutations present only in wild type are shown in bold italic type.
  5. 17 See Figure S3.