Figure 3
From: Gene silencing in HIV-1 latency by polycomb repressive group
Induction of HIV-1 gene silencing by polycomb group proteins. A) Expression levels of EED, one of PRC2 components, were measured by western blot. Thirty ug of nuclear extract were also used. The antibodies were indicated on the right. B) The expression levels of polycomb group proteins, BMI1 and RING2, were examined by western blot. The right panel showed coimmunoprecipitation experiment, immunoprecipitated with RING2 antibody and blotted with BMI1 antibody. C) Ubiquitylation levels of all cell lines were investigated by western blot. Using 30 ug of nuclear extract and antibody against ubiqutylated histone H2A. Anti-laminB was used as a loading control.