Figure 2
From: Gene silencing in HIV-1 latency by polycomb repressive group
Gene silencing by polycomb group-proteins in NCHA cells for HIV-1 latency. A) Methylation levels of histone H3 on the specific lysine residues were measured by western blot. In each lane, 30 ug of nuclear extract were used and the antibodies were indicated on the right. Anti-laminB was used as a loading control. B) Global distributions of histone modifications were examined by ChIP-Seq. Promoters were the regions of 1 kb upstream and downstream of the transcription start site, and gene body was defined as the length from 1 kb downstream of the transcription start site to transcription end site and the remaining regions were intergenic regions. C) The H3K9me3 profile was shown on HIV-1 provirus genome. The X axis represents the HIV-1 genomic position and the Y axis indicates the normalized tag count which is calculated by total tag numbers detected in a 1 kb window divided by total sequenced tag number.