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Figure 5 | Virology Journal

Figure 5

From: The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene-silencing mechanism in tomato plants

Figure 5

Role of ToLCKeV AC3 on gene-silencing. (a) High level transcription of a part of PCNA gene lead to the reduced growth of the plant. Retardation is observed in the growth of the plant agroinoculated with the wild type VIGS vector (wild type AC3) along with PCNA fragment under 35S promoter. Growth retardation is evident in this experiment by shortened height and decreased internodal distance between the stems of the tomato plant (plant on the left) and the plant agroinoculated with AC3 mutated VIGS vector (middle), control vector without any geminiviral DNA (right) and plant without any agroinoculation (not shown). (b) Growth retardation was coupled with reduced flowering (circle) and no fruits. (c) Normal flowering (circles) and developing fruits (arrow) were observed in plant agroinoculated with AC3 mutated VIGS vector. (d) Leaf morphology was altered in the plants agroinoculated with wild type VIGS vector.

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