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Figure 4 | Virology Journal

Figure 4

From: The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene-silencing mechanism in tomato plants

Figure 4

Semi-quantitative amplification of episomal DNA from wild-type and AC3 mutated replicon. Tomato plant leaves were infiltrated with wild-type replicon (pCK2) and AC3 mutated replicon (pCK2M21) separately. DNA from the infiltrated leaves was isolated at 5, 10 and 15 days post infiltration and subjected to Dpn I restriction digestion. Equal quantities of DNA were then used to amplify episome or actin. PCR conditions were specific to amplify only a part of replicon from the episome. Difference in the amplification of replicon in wild-type and mutant was prominent in the 10 dpi sample (3-4 folds difference). By 15 dpi, the difference in the amplification of episome was only 1.5-2 folds.

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Virology Journal

ISSN: 1743-422X