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Figure 2 | Virology Journal

Figure 2

From: The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene-silencing mechanism in tomato plants

Figure 2

Mutations in ToLCKeV AC3. (a) Schematic diagram of the CR-AC3 region of the replicon constructs. The hyphenated arrows indicate the mutations in AC3 ORF. Numbers indicate the base positions with reference to the viral genome. (b) Sequence alignment of the AC3 mutated at start codon with the wild type AC3. The mutated base is shown against the white background at base number 2 in the AC3 ORF. Mutation was confirmed by sequencing. This mutant construct is denoted as CR-AC3M. (c) Termination mutations in AC3 were located at bases 62 and 64 of the AC3 ORF (denoted as CR-AC3M21). Details of these mutations have been explained in the text.

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