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Figure 1 | Virology Journal

Figure 1

From: The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene-silencing mechanism in tomato plants

Figure 1

Viral replicon construction in yeast. Schematic diagram representing the construction of viral replicon in yeast. YCp50 is a binary plasmid that is capable of replication in bacteria and yeast. ARS and CEN4 sequences of the plasmid confer the ability to replicate in yeast. Removal of ARS fragment renders the plasmid unable to replicate in yeast (YCpO-). CR-AC3 fragment of the begomovirus contains the cis-acting sequences (origin of replication) and trans-acting viral genes (AC1, AC3) required for viral replication. Cloning of CR-AC3 of MYMIV at Hind III site was reported to confer YCpO- the ability to replicate in yeast.

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Virology Journal

ISSN: 1743-422X