Primers
|
Position
|
Sequence(5'-3')
|
Fragment
|
---|
S1 b
|
Used for 5'-RACE
|
CATGGCTACATGCTGACAGCCTA
|
5'RACE
|
S2
|
344-367
|
TGAAAGGCGGGCGCTTGGTGACA
|
5'RACE
|
U1
|
379-399
|
TAAgTTTTACCGTCGTTCCC
|
UFR
|
U2
|
718-736
|
ACCGAGCGTGGAGTCAAT
|
UFR
|
A1
|
524-541
|
CGGAAGTAAAACGGCACA
|
A
|
A2
|
2276-2293
|
GATTTCCAACAGCGGTCA
|
A
|
B1
|
2217-2233
|
CGTGTTTGGCAGCCTCAT
|
B
|
B2
|
4700-4717
|
CCACGGGTTCAGGTCTCG
|
B
|
C1
|
4576-4593
|
AACGGCCCAAGCAAGTAT
|
C
|
C2
|
6599-6616
|
TGGAAACGCACGAGCAGT
|
C
|
D1
|
6205-6222
|
GGCAGAGCCATGACAGAC
|
D
|
D2
|
7458-7475
|
GGGTGGAAGCCAAACTCT
|
D
|
GSP1
|
7446-7463
|
GGTGTTTCGCACAGAGTT
|
3'RACE
|
R1
|
3'end
|
TAAgCAAgCATgCCATATgTT
|
3'RACE
|
3' RACERTc
|
Used for 3'-RACE
|
TAAgCAAgCATgCCATATg(T)15
| |
- a The primers used in this study were designed based on JS/CHA/05 (Accession NO. EF149009)
- b S1 was used in 5'-RACE for amplification of the 5' end of RNA.
- c 3'RACERT was used in 3'-RACE for amplification of the 3' end of RNA.