Table 1 Primers for this study

F-L-LL

5'-GTATTGTCAggagctgtgaAGattgacacagcccAAAGCTTGCTGCACAG-3'

PCR mutagenesis for mutant L-LL

R-L-LL

5'-CTGTGCAGCAAGCTTTgggctgtgtcaatCTtcacagctccTGACAATAC-3'

F-L-RR

5'-GTATTGTCAggagctgtgatcattCTcacagcccAAAGCTTGCTGCACAG-3'

PCR mutagenesis for mutant L-RR

R-L-RR

5'-CTGTGCAGCAAGCTTTgggctgtgAGaatgatcacagctccTGACAATAC-3'

F-L-RL

5'-GTATTGTCAggagctgtgaAGattCTcacagcccAAAGCTTGCTGCACAG-3'

PCR mutagenesis for mutant L-RL

R-L-RL

5'-CTGTGCAGCAAGCTTTgggctgtgAGaatCTtcacagctccTGACAATAC-3'

F-S-LL

5'-GCCTTGACATTTGTATTGTCAggagctgtgatcattctgtgtcgggAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant S-LL

R-S-LL

5'-GTTTCTGTGCAGCAAGCTTTcccgacacagaatgatcacagctccTGACAATACAAATGTCAAGGC-3'

F-S-RR

5'-GCCTTGACATTTGTATTGTCAccacgacacaagattgacacagcccAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant S-RR

R-S-RR

5'-GTTTCTGTGCAGCAAGCTTTgggctgtgtcaatcttgtgtcgtggTGACAATACAAATGTCAAGGC-3'

F-S-RL

5'-GCCTTGACATTTGTATTGTCAcccgacacagattctagtgtcgaggAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant S-RL

F-S-RL

5'-GTTTCTGTGCAGCAAGCTTTcctcgacactagaatctgtgtcgggTGACAATACAAATGTCAAGGC-3'

F-EX

5'-GCCTTGACATTTGTATTGTCAtggaggcgtgggtacagccccgccccaAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant EX

R-EX

5'-GTTTCTGTGCAGCAAGCTTTtggggcggggctgtacccacgcctccaTGACAATACAAATGTCAAGGC-3'

F-D1

5'-GACATTTGTATTGTCAgagctgtgatcattgacacagccAAAGCTTGCTGCACAG-3'

PCR mutagenesis for mutant D1

R-D1

5'-CTGTGCAGCAAGCTTTggctgtgtcaatgatcacagctcTGACAATACAAATGTC-3'

F-D2

5'-GACATTTGTATTGTCAgctgtgatcattgacacagcAAAGCTTGCTGCACAG-3'

PCR mutagenesis for mutant D2

R-D2

5'-CTGTGCAGCAAGCTTTgctgtgtcaatgatcacagcTGACAATACAAATGTC-3'

F-D3

5'-GCCTTGACATTTGTATTGTCActgtgatcattgacacagAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant D3

R-D3

5'-GTTTCTGTGCAGCAAGCTTTctgtgtcaatgatcacagTGACAATACAAATGTCAAGGC-3'

F-D4

5'-GCCTTGACATTTGTATTGTCAtgtgatcattgacacaAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant D4

R-D4

5'-GTTTCTGTGCAGCAAGCTTTtgtgtcaatgatcacaTGACAATACAAATGTCAAGGC-3'

F-D5

5'-GCCTTGACATTTGTATTGTCAgtgatcattgacacAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant D5

R-D5

5'-GTTTCTGTGCAGCAAGCTTTgtgtcaatgatcacTGACAATACAAATGTCAAGGC-3'

F-D6

5'-GCCTTGACATTTGTATTGTCAtgatcattgacaAAAGCTTGCTGCACAGAAAC-3'

PCR mutagenesis for mutant D6

R-D6

5'-GTTTCTGTGCAGCAAGCTTTtgtcaatgatcaTGACAATACAAATGTCAAGGC-3'

Qst

5'-GAGTGACGAGGACTCGAGCGCATGCTTTTTTTTTTTTTT-3'

RT for cDNA preparation

F-6

5'-GTATAGGTGTTGGCTCTATGC-3'

(-) gRNAs analysis

R-683

5'-GGAGCGGCAGGTTGGTTAACACG-3'

(-) gRNAs analysis

F-12

5'-GTGTTGGCTCTATGCCTTGAC-3'

(-) gRNAs analysis, sg mRNA7 analysis

R-343

5'-ATAGAATAGGCCCAGCACCCC-3'

(-) gRNAs analysis

R-15284

5'-CTCCACAGTGTAACTTATCCTCC-3'

sg mRNA7 analysis

F-actin

5'-CCCATCTATGAGGGCTACGC-3'

β-actin analysis

R-actin

5'-TTTGATGTCACGCACAATTTC-3'

β-actin analysis