Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells

Table 2 Synthetic oligonucleotides used for qRT-PCR

Primers/probes	Oligonucleotide sequences(5'-3')	Nucleotide positions	Gene	Fragment length
FP	ACAAAACACGGACCCGACTT	3977-3996	2B	67 bp
RT	CTTTTACTCCTATGGCCAGTTCCT	4020-4043
Probe	HEX-AACCGCCTGGTGTCCGCGTTT-BHQ1	3998-4018
FP	GAACACATTCTTTACACCAGGAT	7184-7206	3D	121 bp
RT	CATATCTTTGCCAATCAACATCAG	7281-7304
Probe	FAM-ACAACCTACCGCCGAGCCAATTC-TAMRA	7254-7276
FP	AAGGCCATCACCATCTTCCA	127-147	GAPDH	87 bp
RT	GCCAGTAGACTCCACAACATAC	192-213
Probe	FAM-AGCGAGATCCCACCAACATCAAATGGG-BHQ1	149-175

ISSN: 1743-422X