Figure 8
From: Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells
Growth of FMDV wt and FMDV-Ops in BHK-21 cells maintained in the presence and absence of 20 mM ammonium chloride. Confluent BHK-21 cells in 12-well plates were infected with FMDV wt, FMDV-Op8 and FMDV-Op48 at equal amounts of viral RNAs and then maintained with or without 20 mM ammonium chloride. Cell samples were collected every 6 h from 0 h post-infection (h.p.i.) till 30 h.p.i. and then total RNAs were extracted and quantified via qRT-PCR. All the cell samples were tested in triplicate.