Figure 2

Treatment of BHK-21 cells with different concentrations of ammonium chloride. 1 × 10⁶ BHK-21 cells were infected with FMDV at a m.o.i. of 0.01 PFU/cell and maintained in 5% FBS MEM without (0 mM) or with different concentrations of ammonium chloride (10, 15, 20, 30, 40 mM ) for 48 h, respectively. After removing the alkalescent mediums, infected cells kept in fresh MEM with 10% FBS for 24 h followed by single-cell clone selection and cell counting. (A). The number of cells survived the cytolytic infection maintained in the absence or presence of ammonium chloride. (B). The viral genome RNA positive rates of BHK-21 cells survived. 50 single-cell samples were isolated from each cell suspension survived (300 in total), lysed and run on one-step qRT-PCR assays for detection of viral positive-stranded RNAs. The assay was repeated once. (C). The number of positive cells under the treatment of different concentrations of ammonium chloride.