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Table 1 Primers used for PCR amplification and sequencing of GCRV target gene in this study

From: Functional investigation of grass carp reovirus nonstructural protein NS80

Primer Sequence Restriction enzyme
For cloning   
Y2H-NS80-F 5'-AAAGAATTC ATGGCACGCCGCATTACT-3' Eco RI
Y2H-NS80-R 5'-AAAGGATCC TTACAGCAGCAGGGAGGC-3' Bam HI
Y2H-NS38-F 5'-AAAGAATTC GCTTACCGATTGACAACC-3' Eco RI
Y2H-NS38-R 5'-AAAGGATCC ACACCCTTACATACCC-3' Bam HI
Y2H-VP4-F 5'-AAACATATG ATCACCATTGTGGTTATT-3' Nde I
Y2H-VP4-R 5'-AAACCCGGG CTCAAACCCCGGTCGAG-3' Sma I
Y2H-VP6-F 5'-AAACATATG GCACAGCGTCAGTTTTTCG-3' Nde I
Y2H-VP6-R 5'-AAACCCGGG TTAGACGAACATCGCCTG-3' Sma I
For sequencing   
T7 5'-TAATACGACTCACTATAGGGC-3'  
3' DNA-BD 5'-ATCATAAATCATAAGAAATTCGCC-3'  
3' AD 5'-AGATGGTGCACGATGCACAG-3'  
NS80-walking 5'-ATCTCGCTCCAGCACCCT-3'  
VP4-walking 5'-ACTACTACCTCCAGTGGC-3'  
  1. F, forward primer; R, reverse primer.