Table 1 Primers used for PCR amplification and sequencing of GCRV target gene in this study

For cloning

Y2H-NS80-F

5'-AAAGAATTC ATGGCACGCCGCATTACT-3'

Eco RI

Y2H-NS80-R

5'-AAAGGATCC TTACAGCAGCAGGGAGGC-3'

Bam HI

Y2H-NS38-F

5'-AAAGAATTC GCTTACCGATTGACAACC-3'

Eco RI

Y2H-NS38-R

5'-AAAGGATCC ACACCCTTACATACCC-3'

Bam HI

Y2H-VP4-F

5'-AAACATATG ATCACCATTGTGGTTATT-3'

Nde I

Y2H-VP4-R

5'-AAACCCGGG CTCAAACCCCGGTCGAG-3'

Sma I

Y2H-VP6-F

5'-AAACATATG GCACAGCGTCAGTTTTTCG-3'

Nde I

Y2H-VP6-R

5'-AAACCCGGG TTAGACGAACATCGCCTG-3'

Sma I

For sequencing

T7

5'-TAATACGACTCACTATAGGGC-3'

3' DNA-BD

5'-ATCATAAATCATAAGAAATTCGCC-3'

3' AD

5'-AGATGGTGCACGATGCACAG-3'

NS80-walking

5'-ATCTCGCTCCAGCACCCT-3'

VP4-walking

5'-ACTACTACCTCCAGTGGC-3'