Alignment of GCRV NS80 with its homologous proteins. (A) Blastp analysis of GCRV NS80. GSRV (golden shiner reovirus), AGCRV (American grass carp reovirus), CSRV (chum salmon reovirus), SBRV (striped bass reovirus). ' × ' indicates incompatible alignment by accident. 'Red, pink, green, blue, and black' colours represents alignment scores of '≥200, 80-200, 50-80, 40-50, and <40', respectively. (B) Prediction of GCRV NS80 coiled-coil regions by Multicoil program. The x-axis and y-axis displays amino acid position and coiled-coil probabilities, respectively.