Figure 2

Dose-dependent inhibition of HCVpp of 3a and 1a genotype with lysosomotropic agents. HCVpp were produced in HEK 293 T cells and collected in media after filtration in 0.45 micron filter. Huh-7 cells were incubated in the presence or absence of Lysosomotropic agents such as Chloroquine and NH₄Cl at 37°C for 30 min. After 30 min Huh-7 cells were infected with HCVpp of 3a and 1a genotype in the presence or absence of different concentrations of lysosomotropic agents and incubated for additional 24 h. After 24 h cells were lysed and luciferase activity was determined by using a luminometer. Luciferase activity is not reported as an absolute value, but is calculated relative to the 'no drug' condition and reported on the y-axis as a percentage. Results are represented as the average and standard error for three independent experiments. (a) Dose-dependent inhibition of Chloroquine against HCVpp of 1a and 3a genotype. (b) Dose-dependent inhibition of NH₄Cl against HCVpp of 1a and 3a genotype. P value > 0.05 vs control was considered as statistically significant.