Toxicological study of Chloroquine and NH
Cl in Huh-7 cells: Huh-7 cells were plated at the density of 2 × 104 in 96 well plates. After 24 h cells were treated with different concentrations of chloroquine and NH4Cl and control consisted of solvent in which compound dissolved. After 24 h incubation period add MTT solution to all wells and incubated for 3-4 h at 37°C. Viable cells convert MTT to purple formazan crystal. Added DMSO to dissolve the formazan crystals and read absorbance at 570 nm and 620 nm. (a) Toxicological analysis of Chloroquine in Huh-7 cells through MTT cell proliferation assay. (b) Toxicological analysis of NH4Cl in Huh-7 cells through MTT cell proliferation assay.