Figure 4

Protein immunoblot assay for detection of viral proteins. Fiber, E1A, ADP levels were detected upon infection with RL-07 or wtHAdV-5 at various time points p.i.. Actin was used as loading control. Cells were seeded o/n in a 6-well plate and infected with MOI = 1. Cells were harvested 24, 40, 48, 64 and 70 hrs p.i. in Ripa buffer. Protein amounts were determined by BCA protein assay. In each lane 50 μg of protein was loaded. C-mock infected control; W- infected with wtHAdV-5; R- infected with RL-07.