Relative survival as determined by WST-1 assay. Cytopathic activity of RL-07 (grey bar) compared to wtHAdV-5 (black bar) is higher on glioma cell lines U251 and SNB19. Error bars represent the SD (n = 3). * - statistical significance; unpaired, two-sided t-test, p < 0.01. Cells were seeded in 96-well plates 1 day before infection with RL-07 and wtHAd5 (MOI range 0-100 pfu/cell). Viability of the cultures was determined 4 days p.i by WST-1 assay. Very similar results were obtained in a repeat experiment.