Identification of a spliced gene from duck enteritis virus encoding a protein homologous to UL15 of herpes simplex virus 1

Table 2 Primers used in the this study

Primer name	Primer sequences (5' to 3')	Note
P15f	ATAGGATCC ATGTTCGGGGCAACTTTCG	UL15 forward/Bam HI^a
P15r	GGCAAGCTT TGGTACATATCCTACGTGCGC	UL15 reverse/Hin dIII^a
Psjf	TTGAATTATTCCAGAAGATGA	Splice junction forward
Psjr	TTTCTTGCATAAATGAATCG	Splice junction reverse
P898f	AATTTGGGCAACTCAATGGTT	Northern probe forward
P898r	TCTGCCGTACGTCTCATAGC	Northern probe reverse
P15-5	TGGTGCATGCTCGCAACAGT	UL15 5'-RACE primer
P15.5-5	TGTTCCAACCCGTATATTAC	UL15.5'-RACE primer
P15-3	TTACCACTGCGTGCCTCCCA	UL15 3'-RACE primer
Paf	GCGCTCGTTGTTGACA	β-actin forward
Par	TCATCCCAGTTGGTGACA	β-actin reverse

^aThe incorporated Bam HI and Hin dIII enzyme sites within P15f and P15r are underlined within primer sequences respectively.

ISSN: 1743-422X