The result of splice junction mapping. (A) The RT-PCR product obtained with primers Psjf and Psjr in DEV-infected CEF cells. The primers were expected to yield a fragment of 786 bp as the spliced UL15 product. DNA marker fragments are shown at the right (in bp). (B) Splice sites within the UL15 gene. Intron/exon boundaries are in bold and the splice sites are indicated by vertical arrows. Exon sequences are in uppercase and intron sequences are in lowercase. A number within brackets in the intron sequence is the number of nucleotides omitted. The mature mRNA fragment near the splice site and the corresponding amino acid residues are shown in the box and the sequencing result of the RT-PCR product is indicated below.