Table 1 Sequences of primers and target siRNAs
From: Role of HCV Core gene of genotype 1a and 3a and host gene Cox-2 in HCV-induced pathogenesis
Primers used in vector construction | |
|---|---|
Core3a-sense | ATGAGCACACTTCCTAAACC |
Core3a-antisense | CATGGCTGCTGGATGAATTA |
3aCore-sense | GCGATATCATGAGCACACTTCCTAAA |
3aCore-antisense | AATCTAGATCATGGCTGCTGGATGAAT |
1aCore-sense | GCGATATCAGCACGAATCCTAAAGGT |
1aCore-antisense | AATCTAGATTAGG CTGAAGCGGGCACGGT |
Primers used in Real-Time PCR | |
RTp3.1-sense | GGACGACGATGACAAGGACT |
RT1a-antisense | GGGGAGACAGGA GCCATC |
RT3a-antisense | GGCTGTGACCGTTCAGAAGT |
Cox-2-sense | AACCCACTCCAAACACAG |
Cox-2-antisense | CTGGCCCTCGCTTATGATCT |
iNOS-sense | CACCTTGGAGTTCACCCAGT |
iNOS-antisense | ACCA CTCGTACTTGGGATGC |
VEGF-sense | CTTGCCTTGCTGCTCTACC |
VEGF-antisense | CACACAGGATGGCTTGAAG |
GAPDH-sense | ACCACAGTCCATGCCATCAC |
GAPDH-antisense | TCCACCACCCTGTTGCTGTA |
siRNAs used for silencing | |
Csi27-sense | AAGGATGGTGTTTCTTTTGGTCCTGTCTC |
Csi27-antisense | AAACCAAAAGAAACACCATCCCCTGTCTC |
Csi352-sense | AAATCGATGACTTTACCCAAACCTGTCTC |
Csi352-antisense | AATTTGGGTAAAGTCATCGATCCTGTCTC |
Scramble-sense | AAGTCGAGTCGCGTATGCAGGCCTGTCTC |
Scramble-antisense | AACCTGCATACGCGACTCGACCCTGTCTC |
COXsi-sense | AATTATTTCTGAAACCCACTCCCTGTCTC |
COXsi-antisense | AAGAGTGGGTTTCAGAAATAACCTGTCTC |