Silencing of Cox-2 and HCV 3a Core genes either alone or in combination and reduction of viral titer in Huh-7 cells infected with HCV 3a sera. (A) After 3 days of post infection of Huh-7 cells with HCV 3a sera (Ser 3a), cells were transfected with siRNAs (Csi27 and COXsi) either alone or in combinations (Csi27+COXsi). Data are expressed as mean percent viral load of non-siRNA treated samples. (B) Gene expression analysis was performed using Real-Time PCR. The effect of siRNA on the induction of genes in serum infected and siRNA treated Huh-7 cells. Data are expressed as relative fold reduction to mock sample for Cox-2, iNOS and VEGF genes. (C) The effect of siRNA on protein expression levels of Cox-2, VEGF, p-Akt and GAPDH were determined after 72 hrs post infection with HCV 3a Serum by Western blot analysis from Huh-7 cell lysates using specific antibodies. Three independent experiments with triplicate determinations were performed. Error bars indicate, mean S.D. *#p < 0.001 verses Csi27. * p < 0.001 verses Ser3a.