TLC and HPLC chromatogram of SM Fractions. (A) Ran SM extract in TLC plate. Analyzed the sample at UV light of 254 wavelength. It separates into four components S1, S2, S3, and S4 in solvent (ethyl acetate: chloroform 60:40) with Rf values 0.51, 0.40, 0.64 and 0.75 respectively. (B) HPLC Chromatogram of S1 fraction. (C) HPLC chromatogram of S2 fraction.