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Figure 4 | Virology Journal

Figure 4

From: The duck hepatitis virus 5'-UTR possesses HCV-like IRES activity that is independent of eIF4F complex and modulated by downstream coding sequences

Figure 4

The DHV-1 IRES does not require intact eIF4G. (A) Flexi rabbit reticulocyte lysates were preincubated with or without recombinant FMDV L protease at a final concentration of 0, 1, 5, 25 μg/ml prior to the individual addition of the dicistronic capped RNAs indicated in the figure and translation of the reporter proteins was evaluated. (B) Western-blot showing the effect of recombinant FMDV L protease addition on eIF4GI.

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