Figure 3

SDS-PAGE analysis of supernatant or inclusion bodies after ultrasonic disruption. Lane M: Protein Markers; lane 1: Supernatant after ultrasonic disruption of the tD4-producing cells that used binding buffer without the compounds; lane 2: Precipitation after ultrasonic disruption of the tD4-producing cells that used binding buffer without the compounds; lane 3: Supernatant after ultrasonic disruption of the tD4-producing cells that used binding buffer containing the compounds β-mercaptoethanol, urea, Tween 20, glycerol, and SDS; lane 4: Precipitation after ultrasonic disruption of the tD4-producing cells that used binding buffer with the compounds; lane 5: Supernatant after ultrasonic disruption of the tD5-producing cells that used binding buffer without the compounds; lane 6: Precipitation after ultrasonic disruption of the tD5-producing cells that used binding buffer without the compounds; lane 7: Supernatant after ultrasonic disruption of the tD5-producing cells that used binding buffer with the compounds; lane 8: Precipitation after ultrasonic disruption of the tD5-producing cells that used binding buffer with the compounds.