Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Effect of compounds on the purification and antibody preparation of the extracellular domain fragment of the receptor CD163

Figure 2

PCR amplification of the tD4 and tD5 genes of CD163 and the restricted enzymatic digestion of pET-28a-tD4 and pET-28a-tD5. (a) PCR amplification of the tD4 and tD5 genes of CD163: Lane M: DL2000 DNA Markers; lane 1: tD4 PCR product (1742 bp); lane 2: tD5 PCR product (1427 bp); lane 3: negative control PCR product. (b) Restriction enzyme digestion of the pET-28a-tD4 and pET-28a-tD5 plasmids: Lane M1: λ-EcoT14 I digest DNA Markers; lane M2: DL2000 DNA Markers; lane 1: Enzyme digestion of pET-28a-tD4 (5354 bp + 1728 bp); lane 2: Enzyme digestion of pET-28a-tD5 (5354 bp + 1413 bp).

Back to article page