Figure 2From: Effect of compounds on the purification and antibody preparation of the extracellular domain fragment of the receptor CD163PCR amplification of the tD4 and tD5 genes of CD163 and the restricted enzymatic digestion of pET-28a-tD4 and pET-28a-tD5. (a) PCR amplification of the tD4 and tD5 genes of CD163: Lane M: DL2000 DNA Markers; lane 1: tD4 PCR product (1742 bp); lane 2: tD5 PCR product (1427 bp); lane 3: negative control PCR product. (b) Restriction enzyme digestion of the pET-28a-tD4 and pET-28a-tD5 plasmids: Lane M1: λ-EcoT14 I digest DNA Markers; lane M2: DL2000 DNA Markers; lane 1: Enzyme digestion of pET-28a-tD4 (5354 bp + 1728 bp); lane 2: Enzyme digestion of pET-28a-tD5 (5354 bp + 1413 bp).Back to article page