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Figure 6 | Virology Journal

Figure 6

From: Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro: a possible mechanism of high transmissibility

Figure 6

STAT1 and Caspase 3 protein expression in A549 cells infected with pandemic H1N1 and seasonal H1N1 viruses. The expression of proteins was studied by Western blot analysis. Equal amount of protein (10 μg) from infected A549 cells with the two viruses (pandemic H1N1 and seasonal H1N1) at different post infection time points (4, 8, 16 and 24 hpi) was transferred to nitrocellulose membrane. After treating with respective anti-protein antibodies the membranes were developed using 3, 3'-Diaminobenzidine tetrahydrochloride (DABT) and hydrogen peroxide (H2O2) staining. Lane C: Control A549 cells. Expression of β-actin was used as internal control.

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